trans-Cinnamaldehyde-doped quadripartite biopolymeric films: Rheological behavior of film-forming solutions and biofunctional performance of films

Biofunctional quadripartite films were developed by adding trans-Cinnamaldehyde (CIN) (0.4, 0.8, and 1.6% w/v) into chitosan (CH)/poly(vinyl alcohol) (PVA)/fish gelatin (FG) matrices. Rheological data revealed that all solutions exhibited shear-thinning typical entangle polymers behavior. Mechanical results showed the incorporation of CIN caused a significant increase (p < 0.05) in tensile strength, while decrease was observed elongation-at-break. With increasing content, WVP films, which range 0.785–0.995 g mm/kPa h m2, increased to some extent, whereas water solubility absorption reduced up 47 87%, respectively. Moreover, contact angle analysis inclusion made film more hydrophobic, highest value achieved 86.6 ± 0.75° at θt=10. ATR/FT-IR spectroscopy demonstrated formation Schiff-base intermolecular hydrogen bonds between components' functional groups, enhanced films' thermal stability mechanical properties. X-ray diffraction suggested compatibility among components, changes surface confirmed SEM AFM analyses. Likewise, antimicrobial activity CIN-loaded proved against Staphylococcus aureus, Listeria monocytogenes, Salmonella enteritidis, Escherichia coli. The vitro DPPH radical scavenging (~35.8%) ferric reducing power maximum testing concentration. findings this study CIN-doped could be used as promising inner packaging for food products.